RNAi对小鼠胚胎成纤维细胞中MC1R基因表达的影响

doi:10.11843/j.issn.0366-6964.2014.02.008

Abstract

Abstract:

To study the effect of RNAi on MC1R expression in mouse embryo fibroblasts(MEF). MC1R gene was taken as candidate gene affecting the formation of mammal’s hair color. The aim of the study was to inhibit the expression of MC1R by RNA interference in the MEF. Three pairs of specific siRNA for MC1R gene were designed for RNA interference and transferred into the MEF by reverse transfection. After 48 h, the total RNA and protein were extracted from the MEF, and the relative expression of MC1R mRNA and protein was detected by using QRT-PCR technology and Western bolt. The results showed that the relative expression of MC1R mRNA and MC1R protein in the siRNA1 and siRNA2 groups were significantly lower (P<0.05) than those in the blank control group, but the expression of siRNA3 group was unconspicuous. These tests were successfully inhibited the expression of MC1R in the MEF by RNA interference (reverse transfection) with LipofectamineTM RNAiMate. The expression trendency of MC1R gene and protein are consistent. The results showed that the inhibition effect of siRNA1 and siRNA2 groups were effective, the effective siRNA were screened from three specific siRNAs, can be served as a scientific foundation for the further study of regulation mechanism of MC1R in the process of coat development.

CLC Number:

S814.8

YIN Zhi-hong，WANG En-feng， BAI Rui，PANG Quan-hai. Effect of RNAi on MC1R Expression in Mouse Embryo Fibroblasts[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 218-224.

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Effect of RNAi on MC1R Expression in Mouse Embryo Fibroblasts

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